The Myco4test kit for fumonisin is a 10 minute (two 5 minute incubations) competitive enzyme-linked immunosorbent assay. The microtiter strip well test will detect the quantitative presence of fumonisin in corn and corn products (excluding corn gluten meal and corn gluten feed) in a range of 0 to 6.0 ppm. The principles of the test are based on the ability of free toxin, extracted from the sample, to compete with the enzyme-toxin conjugate (enzyme-labeled toxin supplied in the test kit) for antibody binding sites on the test wells. After a brief washing step, substrate is added to the wells which reacts with the bound enzyme conjugate to develop a blue color. The darker the blue color the lower the levels of toxin. The lighter the blue color the higher the levels of toxin. The test results may be read qualitatively visually or read in a microwell reader at 650 nm to provide quantitative results. The concentration of the toxin is determined by comparison to a standard curve. Up to 38 samples may be run using the 48 well test kit. Due to the speed of the test the maximum amount of wells run at one given time is 24 or two strips of 12. Running the test this way will provide a maximum amount of 19 sample wells. The test kit has been calibrated to a mixture of fumonisin B1, B2 and B3 at 5:2:1 ratios against the kit standard. The test kit and is cross-reactive to fumonisin B1 (97%), B2 (128%) and B3 (83%).

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